PCR Supplies
Sciencell Research Laboratories Telomerase Activity Quantification qPCR Assay Kit, 100 reactions, SYBRGreen dye-based, Hot-start property
Telomerase Activity Quantification qPCR Assay Kit, 100 reactions, SYBRGreen dye-based, Hot-start property
Takara Bio PrimeScript™ RT Reagent Kit (Perfect Real Time)
PrimeScript RT Reagent Kit is designed to perform reverse transcription optimized for real-time RT-PCR. It uses PrimeScript Reverse Transcriptase, which features excellent extension. The kit makes fast, efficient cDNA template synthesis for real-time PCR possible. This kit's protocol is simple and suitable for high throughput analysis. It can be used in combination with real-time PCR reagents such as TB Green Premix Ex Taq II (Tli RNaseH Plus) (Cat. #RR820A/B), TB Green Fast qPCR Mix (Cat. #RR430A/B), TB Green Premix Ex Taq (Tli RNaseH Pluse) (Cat. #RR420A/B), or Probe qPCR Mix (Cat. #RR391A/B) for two step real-time RT-PCR. The optimized protocol for assay can be selected in each assay condition using either TB Green or a probe.
Trilink Bio Technologies Inc CLEANSCRIBE RNA POLYMERASE 10U
NC3835460 CLEANSCRIBE RNA POLYMERASE 10U
New England Biolabs, Inc. WarmStart Colorimetric LAMP 2X Master Mix with UDG – 500 reactions
WarmStart Colorimetric LAMP 2X Master Mix with UDG is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of LAMP and RT-LAMP reactions. The inclusion of dUTP and UDG in the master mix reduces the possibility of carryover contamination between reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow. This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15-40 minutes.
Morwell MD Biosciences EZ PCR MYCOPLASMA TEST KIT
PCR Mycoplasma Test Kit, Designed to Detect The Presence of Mycoplasma Contaminating Biological Materials, Such as cultured Cells, Kit Components: Reaction Mix 200uL, Buffer Solution 1mL, Positive Template Control 20uL
New England Biolabs, Inc. ProtoScript II Reverse Transcriptase – 10000 units
ProtoScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48C, providing higher specificity, higher yield of cDNA and more full-length cDNA product up to 12 kb.
New England Biolabs, Inc. Luna Cell Ready Probe One-Step RT-qPCR Kit – 100 reactions
The Luna Cell Ready Probe One-Step RT-qPCR Kit provides all the necessary components for direct, probe-based RNA detection and quantitation, bypassing the need for RNA extraction and purification. The Luna Cell Ready Probe One-Step RT-qPCR Kit is composed of: 1) the Luna Cell Ready Lysis Module and 2) the Luna Universal Probe One-Step RT-qPCR Kit. Coordinating the actions of DNase I and the Luna Cell Ready Protease, the Luna Cell Ready Lysis Module offers a simple alternative workflow resulting in effective cell lysis, RNA release, and genomic DNA removal simultaneously in a 15-minute protocol. The Lysis Module includes a unique Luna Cell Ready RNA Protection Reagent that maintains RNA integrity during cell lysis. The lysis capacity spans 10 -100,000 cells in a 50 µl lysis reaction. Up to 2 µl of lysate (equivalent to RNA from 0.2 - 4,000 cells) can be transferred into 20 µl downstream RT-qPCR reactions.
Air Science Usa CART-36 Mobile Base Stand, Durable Powder Coated Steel, Open Frame Design, 36 in
The Air Science USA CART-36 Mobile Base Stand provides a sturdy and ergonomic foundation for 36-inch wide Air Science Purair PCR Laminar Flow Cabinets and other compatible lab enclosures. Its robust open-frame design is made of durable powder-coated steel, featuring a lower shelf for extra storage. With mobility options like locking casters, this stand offers flexibility and stability, ensuring secure placement while enabling easy repositioning within the laboratory.
ACROBiosystems E. coiI resDNA Quantitative Kit (qPCR)
Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy. In the context of biopharmaceutical production, regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration, and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.